文拉法辛缓释剂治疗广泛性焦虑症的临床研究

目的探讨文拉法辛缓释剂治疗广泛性焦虑症的临床疗效和安全性。方法将136例广泛性焦虑症患者随机分为文拉法辛缓释剂组68例和阿普唑仑组68例。怡诺思组治疗剂量75~150m g/d,阿普唑仑组为1.2~3.6m g/d,观察时间均为6周。治疗前与治疗第2、4、6周末采用汉密尔顿焦虑量表、焦虑自评量表、副反应量表评定临床疗效和副反应。结果两组疗效相当,差异无显著性（P〉0.05）;阿普唑仑组起效时间快于文拉法辛缓释剂组,但远期疗效差于文拉法辛缓释剂组;怡诺思组副反应明显少于阿普唑仑组（χ2=7.49,P〈0.01）。结论文拉法辛缓释剂治疗广泛性焦虑疗效肯定,安全性高,依从性好,值得临床推广应用。     

11446对新婚夫妇α-地中海贫血筛查结果分析

目的报告11446对新婚夫妇α-地中海贫血（α-地贫）检测结果,了解其基因携带率及分布特征。方法组织受检夫妇抽取静脉血,以平均红细胞体积（MCV）〈79fl为地贫表型阳性指标。对其中2891例表型阳性和1820例表型阴性样品进行α-地贫基因分析。结果22892例筛查对象中检出表型阳性5265例,阳性检出率23％。在表型阳性组中有2891例做了α基因分析,检出3种缺失型α-地贫基因1379例。在表型阴性组中有1820例做了α基因分析,检出3种缺失型α-地贫基因49例,缺失型α-地贫基因携带率为13．05％。常见3种缺失型α-地贫等位基因频率依次是（一一^STA）4．24％、（-α^3.7）1．72％、（-α^4.2）0．87％。临床常见α-地贫的发生率依次为静止型α-地贫4．26％、标准型α-地贫8．57％、HbH病0．22％。结论本地区为地贫高发区,应将基因分析列入筛查项目,为制定地贫干预方案提供科学依据。     

人肠道病毒71型VPO蛋白的原核表达及多克隆抗体制备

目的:原核表达并纯化人肠道病毒71型(EV71)VPO蛋白,免疫豚鼠制备多克隆抗体,并鉴定其用于EV71检测的反应性和特异性.方法:PCR方法扩增VPO基因,构建原核表达质粒pET-VPO并转化大肠杆菌,诱导表达并纯化VPO重组蛋白,免疫豚鼠制备抗VPO多克隆抗体,ELISA方法检测抗VPO抗体效价,细胞免疫荧光和Western blot方法鉴定抗体特异性.结果:VPO重组蛋白在大肠杆菌BL21中高效表达,制备的抗VPO抗体效价为1:106.细胞免疫荧光及Western blot检测结果显示,抗VPO多克隆抗体可以识别原核表达及EV71感染细胞中的VPO蛋白.结论:原核表达了EV71的VPO蛋白并制备出抗VPO多克隆抗体,为EV71的临床诊断、疫苗开发和分子病毒学研究提供了新的研究工具和手段.     

Comparison of embolic agents for varices during transjugular intrahepatic portosystemic shunt for variceal bleeding: Tissue gel or coil?

PurposeWe aimed to compare treatment efficacy, safety and material cost between tissue gel and coil regarding variceal embolization during transjugular intrahepatic portosystemic shunt (TIPS).Materials & Methods: This retrospective study including cirrhotic patients with variceal bleeding treated with TIPS combined with variceal embolization between January 2016 and August 2017. Patients were divided into three groups according to embolic agents used in variceal embolization: tissue gel group (Group A), combination group (Group B), and coil group (Group C). The primary endpoint was 1-year rebleeding rate after TIPS creation. The secondary endpoints included shunt dysfunction, overt hepatic encephalopathy, liver function, and embolic agents-related expense.ResultsA total of 60 patients (30, 10, and 20 in Group A, B, and C) were included. Variceal rebleeding occurred in 3 (10%), 0 (0%), and 4 (20%) patients within one year after TIPS creation in Group A, B, and C, respectively. Stent dysfunction occurred in 2 (3.3%) patients and 9 (15.0%) patients experienced overt hepatic encephalopathy. No significant differences were observed between three groups regarding primary and secondary endpoints except embolic agents-related expense, with a significantly lower cost in Group A when compared to the other two groups. Stent dysfunction occurred in two patients, with one patient in Group A developed acute occlusion caused by thrombus and another patient in Group C underwent stent stenosis during follow-up.ConclusionsCompares to coil alone or combines with coil, tissue gel has similar treatment efficacy and safety, but with significantly lower cost for variceal bleeding during TIPS.     

Ruxolitinib add-on in corticosteroid-refractory graft-vs-host disease after allogeneic stem cell transplantation: Results from a retrospective study on 38 Chinese patients

BACKGROUND Graft-vs-host disease (GVHD) is a major cause of mortality after allogeneic hematopoietic stem cell transplantation.Some patients have steroid-refractory(SR) GVHD.AIM To evaluate the effect and safety of ruxolitinib add-on in the treatment of patients with SR acute (a) and chronic (c) GVHD.METHODS We retrospectively analyzed 38 patients administered ruxolitinib add-on to standard immunosuppressive therapy for SR-aGVHD or SR-cGVHD following allogeneic hematopoietic stem cell transplantation.Ruxolitinib was administered5-10 mg/d depending on disease severity,patient status,and the use of antifungal drugs.Overall response rate,time to best response,malignancy relapse rate,infection rate,and treatment-related adverse events were assessed.RESULTS The analysis included 10 patients with SR-aGVHD (gradeⅢ/Ⅳ,n=9) and 28patients with SR-cGVHD (moderate/severe,n=24).For the SR-aGVHD and SRcGVHD groups,respectively:Median number of previous GVHD therapies was 2(range:1-3) and 2 (1-4);median follow-up was 2.5 (1.5-4) and 5 (1.5-10) mo;median time to best response was 1 (0.5-2.5) and 3 (1-9.5) mo;and overall response rate was 100%(complete response:80%) and 82.1%(complete response:10.7%) with a response observed in all GVHD-affected organs.The malignancy relapse rates for the SR-aGVHD and SR-cGVHD groups were 10.0%and 10.7%,respectively.Reactivation rates for cytomegalovirus,Epstein-Barr virus,and varicella-zoster virus,respectively,were 30.0%,10.0%,and 0%for the SR-aGVHD group and 0%,14.3%,and 7.1%for the SR-cGVHD group.CONCLUSION Ruxolitinib add-on was effective and safe as salvage therapy for SR-GVHD.     

Magnetic recyclable CoFe2O4@PPy prepared by in situ Fenton oxidization polymerization with advanced photo-Fenton performance

Here we present a magnetic recyclable photo-Fenton catalyst CoFe₂O₄@PPy with uniform morphology and excellent dispersibility prepared via simple in situ Fenton oxidization polymerization. The CoFe₂O₄ core provides good magnetic recyclability for the catalysts as well as the ion source for catalyzed decomposition of H₂O₂ in PPy coating. The optimal catalytic effect can be obtained by adjusting the ratio of CoFe₂O₄ and PPy. Methylene blue, Methyl orange and Rhodamine B (RhB) employed as model pollutants certificated that the catalyst exhibits a wide range of photodegradability. The decoloration rates reach nearly 100% in the photodegradation of 10 mg L⁻¹ RhB after 2 h visible-light irradiation and only low toxicity small molecules are detected by LC-MS. Moreover, the catalytic activity remains after 5 cycles with decoloration rates up to 90%. The degradation measurement in the presence of scavengers of reactive species reveals that the positive holes (h⁺) and hydroxyl radical (·OH) are the main reactive oxygen species in the CoFe₂O₄@PPy system. The performance enhancement may be attributed to the combination of improved Fenton activity by coordinated Fe²⁺ and PPy redox pairs and photo-catalytic activity by broaden adsorption and photo-generated charge separation.

Here we present a magnetic recyclable photo-Fenton catalyst CoFe₂O₄@PPy with uniform morphology and excellent dispersibility prepared via simple in situ Fenton oxidization polymerization.     

High catalytic activity of CuY catalysts prepared by high temperature anhydrous interaction for the oxidative carbonylation of methanol

CuY catalysts were prepared by high temperature anhydrous interaction between NH₄Y zeolite and copper(ii) acetylacetonate Cu(acac)₂ and the activities were measured for the oxidative carbonylation of methanol to dimethyl carbonate under atmospheric pressure. The bulk and surface properties of the as-prepared catalyst were characterized by XRD, H₂-TPR and XPS techniques. The activation atmosphere of the CuY catalyst and the testing temperature of the catalytic activity was systematically studied. During activation, nitrogen promotes the auto-reduction of Cu²⁺ to form the Cu⁺ active center, but deposited carbon on the surface of the CuY catalyst covers the active center, even plugging the channel, resulting in lower catalytic activity. Oxygen eliminates deposited carbon, but is not so good for the auto-reduction of Cu²⁺. Nitrogen doped with a small amount of oxygen not only eliminates the deposited carbon, but also promotes the auto-reduction of Cu²⁺ to form more Cu⁺ active centers. With the testing temperature increasing, the catalytic activity increases first and then decreases. When the testing temperature is 170 °C, the CuY catalyst with satisfactory activity and stability showed an excellent catalytic activity with 525.1 mg g⁻¹ h⁻¹ space time yield of DMC (STY_DMC) and 18.9% methanol conversion. Then the longevity was investigated at 170 °C for 150 h. During the initial reaction period of 40 h, the STY_DMC value was constant. In the next 20 h, the catalytic activity slightly decreased. But in the last 90 h, the catalytic performance is very stable and the STY_DMC value remains 480 mg g⁻¹ h⁻¹. The main cause of deactivation is the growth of the particles.

The CuY catalysts prepared by high temperature anhydrous interaction were activated under different atmospheres and the activities were measured for the oxidative carbonylation of methanol to dimethyl carbonate under atmospheric pressure.     

Catalytic activation of peroxymonosulfate with manganese cobaltite nanoparticles for the degradation of organic dyes

In this work, we report the facile hydrothermal synthesis of manganese cobaltite nanoparticles (MnCo₂O_4.5 NPs) which can efficiently activate peroxymonosulfate (PMS) for the generation of sulfate free radicals (SO₄˙⁻) and degradation of organic dyes. The synthesized MnCo₂O_4.5 NPs have a polyhedral morphology with cubic spinel structure, homogeneously distributed Mn, Co, and O elements, and an average size less than 50 nm. As demonstrated, MnCo₂O_4.5 NPs showed the highest catalytic activity among all tested catalysts (MnO₂, CoO) and outperformed other spinel-based catalysts for Methylene Blue (MB) degradation. The MB degradation efficiency reached 100% after 25 min of reaction under initial conditions of 500 mg L⁻¹ Oxone, 20 mg L⁻¹ MnCo₂O_4.5, 20 mg L⁻¹ MB, unadjusted pH, and T = 25 °C. MnCo₂O_4.5 NPs showed a great catalytic activity in a wide pH range (3.5–11), catalyst dose (10–60 mg L⁻¹), Oxone concentration (300–1500 mg L⁻¹), MB concentration (5–40 mg L⁻¹), and temperature (25–55 °C). HCO₃⁻, CO₃²⁻ and particularly Cl⁻ coexisting anions were found to inhibit the catalytic activity of MnCo₂O_4.5 NPs. Radical quenching experiments revealed that sulfate radicals are primarily responsible for MB degradation. A reaction sequence for the catalytic activation of PMS was proposed. The as-prepared MnCo₂O_4.5 NPs could be reused for at least three consecutive cycles with small deterioration in their performance due to low metal leaching. This study suggests a facile route for synthesizing MnCo₂O_4.5 NPs with high catalytic activity for PMS activation and efficient degradation of organic dyes.

Catalytic degradation of organic dyes via manganese cobaltite nanoparticles-activated peroxymonosulfate.